Product

Catalog Number

Description

Bovine IgG ELISA

F070

This kit is intended to quantitate bovine IgG contamination in various types of samples throughout the purification process. The colorimetric endpoint can be read within three hours on a conventional microtiter plate reader to a sensitivity of <250pg/mL of bovine IgG.

Bovine Plasma Proteins ELISA

F290

Kit is intended for use in determining BPP contamination in products purified from bovine source material. This assay is very sensitive and detects BPP contamination of <500pg/mL.

Bovine Serum Albumin ELISA

F030

This is a very sensitive assay for BSA, with a limit of detection of <250pg/mL. No interference from human, sheep or rabbit albumin has been demonstrated at a 1mg/mL concentration.

Bovine Transferrin ELISA

F120

Kit is intended to quantitate Bovine Transferrin in various types of samples. The limit of detection is less than 200pg/mL.

Goat IgG ELISA

F210

The specificity of the antibodies used in this kit for goat IgG, heavy and light chain, results in an  assay will provide accurate results for goat IgG levels, even in the presence of other animal proteins.  As such this assay is useful in detecting trace contamination by goat IgG in a variety of sample types include transgenic products.

Human IgA ELISA

F165

This kit is specific for the detection and quantitation of hIgA and thus can be used to quantitate hIgA in the presence of hIgG and hIgM. Limit of detection is ~250pg/mL.

Human Total IgG ELISA

F160

The specificity of the antibodies used in this kit, heavy and light chains, ensures that the assay will provide accurate results for total hIgG even in the presence of other animal proteins.

Human IgM ELISA

F170

This kit is specific for hIgM and thus can be used to quantitate IgM in the presence of IgG and IgA.

Human Serum Albumin ELISA

F055

The colorimetric endpoint can be read on a conventional microtiter plate reader to a sensitivity of <200pg/mL of albumin.

Human Transferrin ELISA

F035N

The colorimetric endpoint can be read on conventional microtiter plate readers to a sensitivity of 100pg/mL of human transferrin.

Insulin ELISA

F040

The antibodies in this kit cross react 100% with insulin from human, bovine and porcine. The colorimetric endpoint can be read on conventional microtiter plate readers to a sensitivity of 250pg/mL of insulin.

F280

This assay is intended for those applications requiring much more sensitivity than can be achieved by our standard Insulin ELISA kit, #F040.  The limit of quantitation is less than 25pg/mL.

Mouse Immunoglobulin Subclass ELISA

                          IgG1

IgG2a

IgG2b

IgG

IgM

F045

F046

F047

F049

F090

These kits are intended to quantify specific mouse IgG subclasses from various types of samples. They can be used to measure a mouse immunoglobulin subclass which may leach off an affinity support during elution of the desired product. Kits can also be used to quantitate mouse immunoglobulin levels in serum of other animals administered mouse monoclonal antibodies in research studies

 Protein A ELISA

 Protein A ELISA

Protein A-h ELISA

F400

F050

F050H

Cygnus Technologies offers 3 different ELISA kits for the quantitation of Protein A leachates in purified immunoglobin samples.  Our first two kits, Cat # F050 and F050H have been sold for several years and have proven to be valid for use in detecting both natural and structurally conserved recombinant Protein A.  The newest kit, Cat # F400, released in September of 2007 will better detect unnatural recombinant constructs of Protein A such as GE Healthcare’s MabSelect SuReTM ligand.  Like the F050H assay, the new F400 assay uses a sample denaturation step to overcome sample/product antibody interferences.  Because so many laboratories have successfully validated and are routinely using our earlier kits #F050 & #F050H, we will continue to offer these kits indefinitely.  First time evaluators of Protein A detection methods are encouraged to evaluate the new #F400 kit regardless of which type of Protein A they use.